ABSTRACT
Objective To evaluate the application values of two kinds of single-platform flow cytometric methods,the Volumetric method based on flow sensor and the Trucount method based on Trucount beads,in the counts of T-lymphocyte subsets in peripheral blood of patients after transplantation.Methods The absolute number and percentage of CD4 +,CD8 +,and CD3 + T cells in peripheral blood samples from 107 patients after liver or renal transplantation were determined by the Trucount method and the Volumetric method,respectively,and their results were compared using paired t-test and linear regression analysis.Five samples with low CD3 + counts were selected and the precisions of the absolute number of CD4 +,CD8 + and CD3 + T ceils detected by the Volumetric method were evaluated.Results There was no significant difference in the levels of CD4+,CD4+/CD3+,CD8+,CD8+/CD3+,and CD4+/CD8 + in peripheral blood between the Trucount method and the Volumetric method (P ＞ 0.05),and the linear regression coefficients between them were from 0.9 to 1.1.When the concentration of CD3 + was equal or more than 40/μL,the coefficients of variation (CVs) were below 5.5％ for the Volumetric method.When the concentration of CD3 + was 20/μL,the CVs of CD3 +,CD4 +,and CD8 + were 5.19％,10.28％ and 6.48％,respectively.Conclusion The single-platform method based on flow sensor is accurate and reproducible for counting T-lymphocyte subsets in peripheral blood,which may be used to monitor the immune state of the patients after liver or renal transplantation.
ABSTRACT
Objective:To explore the value of combined detection of PCT and IL-6 in differential diagnosis SIRSin ICU patients.Methods:100 patients with ICU admitted to our hospital from 2013 to 2016 were choosen,including 61 cases with non septic SIRS and 39 cases with sepsis,and 50 healthy persons over the same period were selected as control,and they were divided into non-septic group,sepsis group and control group.The levels of serum PCT and IL-6 were detected by electrochemiluminescence assay,and took PCT of 2 g/L and IL-6 of 50 ng/L for the critical value to identify non infectious SIRS and sepsis,to evaluate the clinical diagnostic value of combined detection.Results:The maximum values of PCT and IL-6in the non-septic group respectively were 0.91 ± 0.54 μg/L and 62.77± 11.75 ng/L,in the septic group respectively were 24.49± 5.00 μg/L and 1542.69± 361.66 ng/L,in the control group respectively were 0.08± 0.06 tμg/L and 3.68± 1.11 ng/L,the maximum values of PCT and IL-6 in the non-sepsis group and the sepsis group were significantly higher than control group (P＜0.05).Compared with the non-septic group,the maximum valuesin sepsis group were significantly increased (P＜0.05).The proportions of PCT ＞ 2 g/L and IL-6 ＜ 50ng/L in the non-septic group respectively were 21.31％ and 65.57％,in the septic group respectively were 92.31％ and 87.18％,the proportions of PCT＞2 g/L,IL-6＜50 ng/L in the sepsis groupwere significantly higher chan those in the non-septic group (P＜0.05).The positive predictive values,sensitivity and specificity of PCT were higher than IL-6,the positive value,specificity of combined detection was higher than IL-6 and PCT,while the sensitivity of combined detection was higher than IL-6,P＜0.05.Conclusions:Combined detection of PCT and IL-6 is helpful for differential diagnosis of sepsis and non-septic SIRS.
ABSTRACT
Objective To study the effect of Golgi protein 73(GP73) on inflammation, and to reveal the effect of GP73 on tumorigenesis and metastasis.Methods The transcriptional activity of NF-κB and the expression of IL-1β, IL-6 and TNF-αwith GP73 overexpression or knockdown were detected to illuminate the role of GP73 in inflammation.According to the TCGA database, the correlation between the transcriptional activity of GP73 and the expression of NF-κB, IL-1β, IL-6 and TNF-αwas analyzed to determine the role of GP73 in tumor inflammation.Results Correlative analysis showed that there was a positive correlation between the expression of GP73 with NF-κB, IL-1β, IL-6 and TNF-α.The transcriptional activity of NF-κB was upregulated by GP73 overexpression, but downregulated by GP73 knockdown.The expression of IL-1β, IL-6 and TNF-αwas upregulated by GP73 overexpression.Ammonium pyrrolidinedithiocarbamate ( PDTC ) was in-volved in inflammation reaction induced by GP73.Conclusion GP73 is possibly involved in inflammation and promotes tu-morigenesis and metastasis.
ABSTRACT
Objective To evaluate the function of MRE11 in inflammasome activation.Methods Different stimuli,in-cluding Poly(I∶C), Poly(dA∶dT),E.coli gDNA,293T gDNA,CPPD and HSV,were used to identify the effective inflamma-some activator using ELISA.Then, MRE11 siRNA oligos were sythesized and transfected into THP-1 cells while Western blotting was used to analyze the efficacy of MRE 11 knockdown .Finally ELISA and Western blotting were used to analyze the involvement of MRE11 in inflammasome activation induced by Poly (I∶C), Poly(dA∶dT), E.coli gDNA and 293T gDNA. Results The IL-1βsecretion and pro-caspase-1 activation which induced by Poly ( I∶C) , Poly( dA∶dT) , E.coli gDNA and 293T gDNA were reduced with different degrees in MRE 11-knockdown THP-1 cells.Conclusion These results indicate that MRE11 is required for inflammasome activation induced by genetic materials .